Publications
At Wortzel Lab, we are committed to advancing the understanding of cancer cell communication through extracellular vesicles (EVs) and their DNA content. Our groundbreaking research sheds light on the intricate mechanisms underlying tumor–immune interactions, anti-tumor immunity, and metastatic progression. By harnessing cutting-edge genomics, imaging, and in-vivo cancer models, we aim to decode EV-DNA biology and utilize this knowledge to enhance early detection methods and develop innovative therapeutic approaches against metastasis.
2026
Tumour-derived Extracellular Vesicle and Particle Reprogramming of Interstitial Macrophages in the Lung Pre-Metastatic Niche Enhances Vascular Permeability and Metastatic Potential
Shani Dror, Inbal Wortzel, Serena Lucotti, Yura Seo, Jianlong Li, Lee Shaashua Berger, Irina Matei, Nancy Boudreau, Haiying Zhang, Maider Astorkia Amiama, Doron Betel, Han Sang Kim, Jai Prakash, Jacqueline Bromberg and David Lyden.
Accepted, Nature Cancer
Activated T cell extracellular vesicle DNA transfer enhances antigen presentation and anti-tumor immunity
Mengying Hu, Diao Liu, Inbal Wortzel, Paul Collier, Theodore M Nelson, Jonathan Foox, Guojie Zhong, Gabriel Tobias, Tetsuhiko Asao, Linda Bojmar, Candia M Kenific, Gang Wang, Simone Caielli, Zurong Wan, Sarah Qureshy, Max Reed, Richard Piszczatowiski, Purnima Ravisankar, Julia A. Brown, Sihan Xiong, Huajuan Wang, Pernille Lauritzen, Yael Ayalon, Henrik Molina, William R. Jarnagin, Moshe Oren, Ben Z. Stanger, Jack Bui, Gabriele Bergers, Agnes Noel, Paul M. Grandgett, Michal A. Hollingsworth, David Tuveson, Nancy Boudreau, Jacquline Bromberg, David Kelsen, David R. Jones, Laura Santamborgio, Melody Y. Zeng, Virginia Pascual, Han Sang Kim, Christopher E. Mason, Irina R. Matei, Haiying Zhang and David Lyden.
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Accepted, Cancer Cell
2024
Unique structural configuration of EV-DNA primes Kupffer cell-mediated antitumor immunity to prevent metastatic progression
Wortzel I, Seo Y, Akano I, Shaashua L, Tobias GC, Hebert J, Kim KA, Kim D, Dror S, Liu Y, Azrak GC, Cioffi M, Johnson KE, Hennika T, Twerski MZ, Kushner A, Math R, Han YD, Han DH, Jung M, Park J, Paik S, Shin JS, Lee MG, Russo MV, Zakheim D, Barnes J, Mehta S, Manova K, Schwartz RE, Thakur BK, Boudreau N, Matei I, Zhang H, Sidoli S, Bromberg J, David Y, Kim HS, Lyden D.
Nat Cancer. 2024. doi: 10.1038/s43018-024-00862-6 PMID: 39627554
Genome-wide methylation profiling reveals extracellular vesicle DNA as an ex vivo surrogate of cancer cell-derived DNA
Kim KA, Kim S, Wortzel I, Lee S, Han YD, Kim TM, Kim HS
Sci Rep. 2024 Oct 15;14(1):24110. doi: 10.1038/s41598-024-75287-3. PMID: 39406948
Tumour-derived Extracellular Vesicle and Particle Reprogramming of Interstitial Macrophages in the Lung Pre-Metastatic Niche Enhances Vascular Permeability and Metastatic Potential.
Dror S, Lucotti S, Asao T, Li J, Wortzel I, Berger LS, Matei I, Boudreau N, Zhang H, Jones D, Bromberg J, Lyden D.
Res Sq [Preprint]. 2024 May 30:rs.3.rs-4462139. doi: 10.21203/rs.3.rs-4462139/v1. PMID: 38853850
Genome-wide methylation profiling reveals extracellular vesicle DNA as an ex vivo surrogate of cancer cell-derived DNA
Kim KA, Kim S, Wortzel I, Lee S, Han YD, Kim TM, Kim HS
Sci Rep. 2024 Oct 15;14(1):24110. doi: 10.1038/s41598-024-75287-3. PMID: 39406948
2023
Quantifying Golgi Apparatus Fragmentation Using Imaging Flow Cytometry.
Wortzel I, Porat Z.
Methods Mol Biol. 2023;2635:173-184. doi: 10.1007/978-1-0716-3020-4_10. PMID: 37074663.
2022
Detection of circulating KRAS mutant DNA in extracellular vesicles using droplet digital PCR in patients with colon cancer.
Choi J, Cho HY, Jeon J, Kim KA, Han YD, Ahn JB, Wortzel I, Lyden D, Kim HS.
Front Oncol. 2022 Dec 15;12:1067210. doi: 10.3389/fonc.2022.1067210. PMID: 36591510
Applying imaging flow cytometry and immunofluorescence in studying the dynamic Golgi structure in cultured cells.
Wortzel I, Porat Z, Seger R, Maik-Rachline G.
STAR Protoc. 2022 Apr 5;3(2):101278. doi: 10.1016/j.xpro.2022.101278. PMID: 35403004
2021
Alternative Splicing of MAPKs in the Regulation of Signaling Specificity.
Maik-Rachline G, Wortzel I, Seger R.
Cells. 2021 Dec 8;10(12):3466. doi: 10.3390/cells10123466. PMID: 34943973;
Mitotic HOOK3 phosphorylation by ERK1c drives microtubule-dependent Golgi destabilization and fragmentation.
Wortzel I, Maik-Rachline G, Yadav SS, Hanoch T, Seger R.
iScience. 2021 May 31;24(6):102670. doi: 10.1016/j.isci.2021.102670. PMID: 34189435
2019
Exosome-Mediated Metastasis: Communication from a Distance
Wortzel I, Dror S, Kenific CM, Lyden D.
Dev Cell. 2019 May 6;49(3):347-360. doi: 10.1016/j.devcel.2019.04.011. PMID: 31063754.
2017
High Throughput Analysis of Golgi Structure by Imaging Flow Cytometry.
Wortzel I, Koifman G, Rotter V, Seger R, Porat Z.
Sci Rep. 2017 Apr 11;7(1):788. doi: 10.1038/s41598-017-00909-y. PMID: 28400563
2015
Mitotic Golgi translocation of ERK1c is mediated by a PI4KIIIβ-14-3-3γ shuttling complex.
Wortzel I, Hanoch T, Porat Z, Hausser A, Seger R.
J Cell Sci. 2015 Nov 15;128(22):4083-95. doi: 10.1242/jcs.170910. PMID: 26459638.
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