Research
At the Wortzel Lab, we investigate how cancer cells communicate with their environment through extracellular vesicles (EVs), with a particular focus on DNA associated with EVs. Our research uncovers how EV-DNA shapes tumor–immune interactions, influences anti-tumor immunity, and affects metastatic progression. By combining advanced genomics, imaging, and in-vivo cancer models, we aim to decode the molecular mechanisms governing EV-DNA biology and leverage this knowledge to improve early detection and develop new therapeutic strategies against metastasis.
EVs carry a wide variety of biomolecules, including DNA. We study the biology and function of EV-associated DNA (EV-DNA) in cancer progression and metastatic defense: how chromatinized DNA is packaged into EVs, how recipient cells detect and respond to it, and how these signals reshape local tissues and systemic immunity.
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Biogenesis of EV-DNA
We investigate the cellular pathways that govern how DNA becomes incorporated into extracellular vesicles. By defining the molecular and structural processes that enable EV-DNA packaging, we aim to uncover fundamental principles of EV formation and cargo selection.
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EV-DNA Sensing and DNA-Damage Response Crosstalk
We study how recipient cells detect EV-associated DNA and how this communication intersects with intrinsic genome-stress and DNA-damage response pathways. Our goal is to understand how EV-DNA signals influence immune activation and tissue adaptation in cancer.
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Chromatin Architecture and Histone Processing in EVs
We explore the chromatin landscape associated with EV-DNA, including nucleosome organization and histone modifications. This work seeks to illuminate how chromatin structure and regulated histone processing contribute to EV function and their impact on tumor–host interactions.